منابع مشابه
Visualizing vesicles
In This Issue In This Issue Monitoring MLCK yosin light chain kinase (MLCK) is a busy protein— it phosphorylates myosin's regulatory light chain (and thus activates myosin) during nonmuscle cell contraction, cytokinesis, stress fiber formation, and motility. In general, MLCK is known to be present at the cellular sites implicated in these events, but the results of Chew et al. (page 543) are so...
متن کاملVisualizing Postendocytic Traffic of Synaptic Vesicles at Hippocampal Synapses
We have investigated mechanisms in postendocytic processing of synaptic vesicles at hippocampal synapses, using synaptobrevin/vesicle-associated membrane protein (VAMP) tagged with variants of the green fluorescent protein. Following exocytosis, VAMP is retrieved at synaptic and adjoining axonal regions. Retrieved VAMP-containing vesicles return to synaptic vesicle clusters at a rate slower tha...
متن کاملVisualizing recycling synaptic vesicles in hippocampal neurons by FM 1-43 photoconversion.
Exo-endocytotic turnover of synaptic vesicles (SVs) at synapses between hippocampal neurons in culture was examined by electron microscopy (EM). We carried out photoconversion (PC) of the fluorescent endocytotic marker FM 1-43 by using 3,3'-diaminobenzidine to convert the dye signal into an electron-dense product. Electron-dense products were located almost exclusively in SVs, whose densities w...
متن کاملVMAT2 binding is elevated in dopa-responsive dystonia: visualizing empty vesicles by PET.
Dopa-responsive dystonia (DRD) is a lifelong disorder in which dopamine deficiency is not associated with neuronal loss and therefore it is an ideal human model for investigating the compensatory changes that occur in response to this biochemical abnormality. Using positron emission tomography (PET), we examined the (+/-)-alpha-[(11)C]dihydrotetrabenazine ([(11)C]DTBZ) binding potential of untr...
متن کاملProperties of ribbon and non-ribbon release from rod photoreceptors revealed by visualizing individual synaptic vesicles.
Vesicle release from rod photoreceptors is regulated by Ca(2+) entry through L-type channels located near synaptic ribbons. We characterized sites and kinetics of vesicle release in salamander rods by using total internal reflection fluorescence microscopy to visualize fusion of individual synaptic vesicles. A small number of vesicles were loaded by brief incubation with FM1-43 or a dextran-con...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2002
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb1563iti4